These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. (1990). ACS Nano. 2012;101(8):28619. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. 4h after microporation, the sizes of the micropore decreased slightly. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Data File Dextran. 2009;301(8):60913. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Hydrodynamic Properties of Tragacanthin. 1994, 95-0355. Epub 2017 Jun 1. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. European Polymer Journal, 42, 1086-1092. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. (2008). Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). Chem. European Polymer Journal, 37, 619-622. The above data are not useful because it can not be compared with those obtained in this work. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Masuelli, Martin Alberto (2013). Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Indo AM J Pharm. 8600 Rockville Pike In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. (2008). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Advanced Drug Delivery Reviews, 60, 1650-1662. Experimental and numerical study on the mixed lubrication Polymers | Free Full-Text | Confinement-Induced Fractionation and California Privacy Statement, Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. 2. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. WebR H Hydrodynamic radius [m] The basic DLS setup. ; Hogan, M.P. Treatment of acne scars by fractional bipolar radiofrequency energy. Carbohydr Polym. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Would you like email updates of new search results? The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Small Intestinal Permeability and Gut-Transit Time Determined International Journal of Biological Macromolecules, 42, 133-137. Dextran Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. 2015;32(5):170413. PMC The authors declare that they have no competing interests. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Dilute solution properties and degree of chain branching for dextran. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Cell permeabilization using a non-thermal plasma - IOPscience FITC-dextran solution was prepared at 1mM in PBS. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. 2013, Article ID 360239, pp. A solvent at this temperature is called a theta solvent [53]. Charge: Anionic. The enhanced permeability through micropores was confirmed by Franz diffusion cell. ; Picout, D.R. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Masuelli, Martin Alberto. The site is secure. 2012;27(3):20212. Naik A, Kalia YN, Guy RH. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Increased TEWL values are evidence of micropores formation. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Proc. Kasaai, Mohammad R. (2012). The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). American Journal of Food Science and Technology 2013, 1(3), 60-66. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Dextran Fractions. Epub 2022 Feb 2. (1937). dextran hydrodynamic radius. Colloid Polym. Dextran. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). statement and 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. 2012;3(1):208. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Carbohydrate Research, 340, 811-826. ; Grishchenko, A.E. They concluded that a power law behavior seems to be approached only in the small molecular weight region. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. Accessibility Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Guner, Ali (1999). We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. For data requests, please contact the authors. Polysaccharide hydrogels for modified release formulations. Google Scholar. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Catiker, E.; Guner, A. government site. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Keywords: FITC dextran; intestinal permeability; transit time. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Synthesis and characterization of blue dextrans One day before the experiment, the skin was stabilized in the skin culture medium. ; Dutt, N.V.K. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Drug Deliv. J Pharm Sci. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Porcine wound models for skin substitution and burn treatment. By using this website, you agree to our Webof overall macromolecular geometry and hydrodynamic draining. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. The Mark-Houwink. Nanoparticles in magnetic resonance imaging: from simple to dual Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Transdermal drug delivery: overcoming the skins barrier function. dextran hydrodynamic radius Journal of Chemical Education, 49(8), 577-579. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. In addition, we evaluated the regeneration of SC after microporation. TEWL measurements were taken 30min after microporation of the human and pig skin. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Biophysical Journal, 87, 4259-4270. sharing sensitive information, make sure youre on a federal Webdextran hydrodynamic radius. eCollection 2018 Feb. Molecules. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Advances in Cosmetic Surgery. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Carbohydrate Polymers 88 373-381. The basic setup of a DLS instrument is shown in Figure 1. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows De Belder, A. N. (2001). Curvale, R.; Masuelli, M.; Perez Padilla, A. (2003). The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Hrabchak C, Flynn L, Woodhouse KA. Human skins (Full-thickness human skin) were supplied by HansBiomed. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. dextran hydrodynamic radius. Curley SA. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. In addition, the permeation rate was higher in pig skin than in human skin. HHS Vulnerability Disclosure, Help For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Kasaai, Mohammad R. (2007). 113, 81-86. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. The genus Weissella: taxonomy, ecology and biotechnological potential. Journal of American Chemical Society, 76 (20), 5041-5052. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. TEWL measurements were performed before and 30min after microporation. All branched polysaccharide types could be described approximately by the hyperbranching theory. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Chem. Exp Cell Res. MeSH Gold MH, Biron JA. dextran The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France).
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